Thermo Scientific™

POROS™ CaptureSelect™ IgA Affinity Column, 4.6 x 100 mm

Catalog number: 4485170
Thermo Scientific™

POROS™ CaptureSelect™ IgA Affinity Column, 4.6 x 100 mm

Catalog number: 4485170
Catalog Number
4485170
Unit Size
1 column
Price (USD)
3,385.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44851701 column
3,385.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ IgA HPLC columns incorporate the advantages of high-speed POROS™ chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three-minute quantitation of IgA.

Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes
Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins, fusion proteins
Hands-off operation—HPLC method can be automated for streamlined operation
Robust—allows for high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ IgA affinity ligands and POROS™ 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid titer determinations and small-scale purification of IgA.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

Specifications

Stationary Phase
CaptureSelect™ IgA
Column Type
Affinity
Form
Pre-packed Column
Product Line
CaptureSelect™, POROS™
Target
Protein
Type
Affinity Column
Matrix
POROS
Diameter (Metric)
4.6 mm
Elution Volume Max
29 column volumes
Elution Volume Min
14 column volumes
For Use With (Equipment)
FPLC
High-throughput Compatibility
Automated Protocols
Max. Operating Pressure
2600 psi, 180 bar
Length (Metric)
100 mm
Particle Size
20 micron

Contents & Storage

Column includes EZ grip fittings and column test certificate.
Store at 2–8°C. DO NOT FREEZE.

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