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Pierce™ Chicken IgY Purification Kit, 1 L - FAQs

Pierce™ Chicken IgY Purification Kit, 1 L - FAQs

View additional product information for Pierce™ Chicken IgY Purification Kit - FAQs (44918)

8 product FAQs found

Is there any difference between IgY from serum versus egg yolks?

No. Serum and egg IgY are the same. The advantage of collecting IgY from the yolks is the high concentration of IgY per yolk versus IgY in serum.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I immobilize IgY on Protein A, Protein G or Protein A/G to immunoprecipitate or affinity purify the antigen?

No. IgY does not bind to Protein A, Protein G or Protein A/G; however, AminoLink Plus Resin (Cat. No. 20501) or Kit (Cat. No. 44894) can immobilize IgY. The AminoLink Plus Resin is aldehyde-activated and reacts with primary amines on the IgY and forms a covalent linkage.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the molecular weight of IgY?

IgY has a mass of 180kDa, which is more than IgG because the IgY heavy chain has an extra domain on the Fc region. The light chains are ~22 kDa and the heavy chains are ~68 kDa; however, estimates vary in the literature.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How should I store the IgY purified using the Thermo Scientific Chicken IgY Purification Kit??

Store the purified IgY at 0-4°C (< 6 months). For longer storage, separate the IgY into small volumes, add a small amount of glycerol (< 10%) to help stabilize the IgY and store at -20°C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much IgY can be purified with the Thermo Scientific Chicken IgY Purification Kit?

The kits can purify up to 100 mg of chicken IgY per yolk (i.e., 500 mg with Cat. No. 44918 and 2,500 mg with Cat. No. 44922).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why do I need to use cold eggs for IgY purification using the Thermo Scientific Chicken IgY Purification Kit?

Delipidation occurs optimally at 4°C, so it is best to have eggs and reagents cold.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do I need to change the protocol when using frozen eggs for IgY purification using the Thermo Scientific Chicken IgY Purification Kit?

To store yolks frozen before purification, mix them well with cold Delipidation Reagent (two times the original yolk volume), and store them for approximately one year.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do I need to use fresh yolks for IgY purification using the Thermo Scientific Chicken IgY Purification Kit?

Eggs stored in the refrigerator for up to two months may be used or yolks can be processed and stored frozen.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.