Melon™ Gel Spin Plate Kit for IgG Screening

Catalog number: 45208

Thermo Scientific™

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Description

The Melon Gel Spin Plate Kit for IgG Screening offers a high-throughput format for quick purification and screening of antibodies. This kit enables purification of up to 96 samples of 20-100 µl in 15 minutes or less, offering a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Features of Melon Gel IgG Purification Resin:

Simple, one-step protocol—IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification—antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity—for many species of serum antibody recovery is greater than 90% with purity greater than 80%
Minimal dilution—reduced need to concentrate antibody before storage or use
Gentle purification—No harsh antibody elution conditions helps retain IgG activity
Regenerable support—Melon Gel Resin can be used for multiple antibody purifications

Melon Gel IgG Purification Kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Related Products
Melon™ Gel IgG Spin Purification Kit
Melon™ Gel Purification Buffer
Melon™ Gel Regenerant
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Kit, 96-well Filter Plate
Column Type: Affinity, Proprietary Resin
Product Size: 2 plates
Immobilized Phase: Proprietary Ligand

Contents & storage

Kit Contents:
• Melon Gel 96-Well Spin Plates, 2 each, contains 100 µL of Melon Gel⁄well (Purification Capacity: 1 mg of human IgG⁄well)
• Melon Gel Purification Buffer, 2 × 100 mL
• Wash Plates (white), 2 each
• Collection Plate

Storage: Upon receipt store at 4°C. Product shipped at ambient temperature.

Documents

Manuals & protocols

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