Pierce™ Streptavidin Coated Plate IP Kit

Catalog number: 45360

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Pierce Streptavidin Coated Plate IP Kit uses coated streptavidin microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

Pierce Coated Plate IP Kits enable rapid immunoprecipitation of multiple samples without the usual tedium of pipetting, centrifuging and separating beaded affinity resin in individual microcentrifuge tubes. Immunoprecipitation is accomplished using coated 96-well microplates rather than beaded agarose resin. The plate format allows for faster processing of multiple samples.

Features of the Streptavidin Coated Plate IP Kit:

• Ready-to-use, high quality coated plates provide high capacity and consistency
• Plate format best suited for simultaneously processing multiple samples and their control conditions
• Faster, easier and more thorough washing than with traditional tube/resin IP methods
• Uses familiar and convenient ELISA tools (multichannel pipettors and plate washing); no tedious separation of supernatant from pelleted resin beads, and no tubes to open and close and centrifuge
• Coated plates are 96-well strip plates, convenient for experiments requiring only a partial plate
• Easy-to-follow instructions, including detailed explanation of appropriate controls

• Rapid, trouble-free immunoprecipitation of multiple samples and their controls
• Immunoprecipitation when plate-based tools and techniques are preferred

Streptavidin is a protein that binds specifically and very strongly to biotin; therefore, the Streptavidin Coated Plate IP Kit (Part No. 45360) is appropriate for immunoprecipitation when using a biotin-labeled (biotinylated) antibody. In fact, this kit can be used to affinity purify a binding partner to any antibody species or subclass or any other protein or molecule that is biotinylated. Because the streptavidin-biotin affinity interaction is so strong, the elution step generally will dissociate only the antigen (binding partner), not the biotinylated antibody or "bait" protein.

Related Products
Pierce™ Protein A/G Coated Plate IP Kit
For Research Use Only. Not for use in diagnostic procedures.


Technique: Pull-Down (Biotin-Based), Immunoprecipitation (IP)
Assay Type: Affinity Purification
Product Size: 2 plate
Target Specificity: Not Target-Specific
For Use With (Equipment): Microplate Mixer

Contents & storage

Sufficient For: Assaying 48 samples with controls or 192 total IP reactions
• Streptavidin Coated 96-well Strip Plates, 2 plates
• Uncoated 96-well Strip Plate (white), 2 plates
• PBS Pack (makes 500 mL), 1 pack
• Biotin Blocking Buffer, 2 x 15 mL
• Surfact-Amps X-100 Detergent Solution, 6 x 10 mL
• Elution Buffer (pH 2.8), 50 mL
• Neutralization Buffer, 12 mL
• Sealing Tape for 96-well Plates, 18 sheets

Store at 4°C.


Manuals & protocols