TRITC (5/6-tetramethyl-rhodamine isothiocyanate), mixed isomer

Catalog number: 46112

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Thermo Scientific TRITC is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Features of TRITC:

Amine-specific labeling—TRITC varieties of rhodamine efficiently label antibodies and other purified proteins at primary amines (lysine side chains)
Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence

Tetramethylrhodamine isothiocyanate (TRITC) is an amine-reactive derivative of rhodamine dye that has wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as western blotting and ELISA.

• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on western blots

Properties of Rhodamine Dyes:
Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.

TRITC is the base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group (—N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules.

Application Data:

Related Products
Pierce™ NHS-Rhodamine Antibody Labeling Kit
NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: TMR (Tetramethylrhodamine), Rhodamine Isomers, TRITC Isomers
Chemical Reactivity: Amine
Solubility: DMF (Dimethylformamide)
Product Size: 10 mg
Excitation⁄Emission (nm): 552⁄575

Contents & storage

Storage: Upon receipt store at -20°C. Product is shipped at ambient temperature. Store fluorescent dye in foil pouch with desiccant to protect from light and moisture.


Manuals & protocols