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CD15 Monoclonal Antibody (MMA), eFluor™ 450, eBioscience™ - FAQs

View additional product information for CD15 Monoclonal Antibody (MMA), eFluor™ 450, eBioscience™ - FAQs (48-0158-41, 48-0158-42)

3 product FAQs found

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.