What kind of buffers and solutions are compatible with peptide clean-up and LC-MS?
Most organic solvents or volatile buffers are compatible with LC-MS. Water and acetonitrile with 0.1% formic acid is the most common solvent system for separation of peptide samples for mass spectrometry analysis. Trifluoracetic acid (TFA) is an alternative ion pairing agent for off-line peptide C18 clean-up or LC-UV analysis. We offer the following LC-MS grade reagents: Pierce 0.1% Trifluoroacetic Acid (v/v) in Water (Cat. No. 85172), Pierce Water, LC-MS Grade (Cat. No. 85189), Pierce Acetonitrile (ACN) (Cat. No. 85188), and Pierce Trifluoroacetic (TFA) (Cat. No. 85183).
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.