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Fluoroskan™ Microplate Fluorometer - FAQs

View additional product information for Fluoroskan™ Microplate Fluorometer - FAQs (5200110)

10 product FAQs found

Does the Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer have different beams for different plate types?

The Fluoroskan Microplate Fluorometer and Fluoroskan FL Microplate Fluorometer and Luminometer have two beam diameter settings. The normal beam with a diameter of 3 mm is generally recommended for 96-well plates and larger wells. The small beam with a diameter of 1.5 mm is recommended for 384-well plates.

How many filters can be installed into the Fluoroskan Microplate Fluorometer?

Eight excitation and eight emission filters can be installed on the Fluoroskan Microplate Fluorometer.

What is the best color plate for fluorescence with a Luminoskan/Fluoroskan microplate reader?

A solid black plate is the best option for fluorescence assays. Black plates with clear bottoms should be used for bottom-reading applications.

How do the fluorescence reference chips, which are installed into the Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer plate carrier work?

The fluorescent chips are installed into the unit to keep the instrument signal level stable. When a filter pair is validated, the instrument measures all four chips in the plate carrier and then automatically selects the most suitable chip. The chip measurement value is stored in the instrument memory. When the measurement is started, autocalibration is performed. The instrument measures this chip during the autocalibration process and compares it with the value in memory. A correction factor is calculated and all further measurement values are multiplied by this factor.

Can the Fluoroskan Microplate Fluorometer be upgraded for luminometric applications?

No, it is not possible to add the luminometric option to the Fluoroskan Microplate Fluorometer.

How do I program the Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer for my Quant-iT assay?

The Online Protocol Library within SkanIt Software contains several pre-written sessions. These can be used as templates and modified for use with other assays.

Which filters ship standard with Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer?

The Fluoroksan Microplate Fluorometer and Fluoroskan FL Microplate Fluorometer and Luminometer ship with two filter pairs:
- Ex 355 and Em 460
- Ex 485 and Em 538
For a complete list of available filters, contact your local Thermo Scientific microplate instrumentation technical support.

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer perform spectral scans?

No, they cannot perform spectral scans. For scanning applications, see Varioskan LUX Multimode Microplate Reader information here: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer read time-resolved fluorescence (TRF) or time-resolved FRET (TR-FRET) assays?

No, they cannot read time-resolved fluorescence (TRF/TR-FRET). For these applications, see Varioskan LUX Multimode Microplate Reader information here: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html.

Can my Fluoroskan Microplate Fluorometer or Fluoroskan FL Microplate Fluorometer and Luminometer read FRET assays?

Yes, they can read any type of fluorescence intensity including FRET assays.