Luminoskan™ Ascent Microplate Luminometer with two dispensers - FAQs

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10 product FAQs found

Which plate types can be used with the Luminoskan Microplate Luminometer, Fluoroskan Microplate Fluorometer, or Fluoroskan FL Microplate Fluorometer and Luminometer?

Plates with 6-, 12-, 24-, 48-, 96- or 384-wells can be measured as long as the plates adhere to Society for Biomolecular Screening (SBS) specification standards.

How many filters can be installed into the Luminoskan Microplate Luminometer?

A maximum of six filters can be installed into the filter wheel positions 1-6.

When is the light shield needed with the Luminoskan or Fluoroskan FL microplate fluorometer?

The light shield should be used with 96- and 384-well plates. Note: If the plates are higher than 15 mm, the light shield must be removed.

What plate colors can be used for luminescence?

For luminometric measurements, white plates are generally used. White plates enhance the signal and avoid possible crosstalk from high signals in neighboring wells.

For the Fluoroskan and Luminoskan readers, when is the adapter for low-profile 384-well plates needed?

For low-profile 384-well plates (plate height less than 13.5 mm), an adapter must be used to raise the plate. The adapter for low-profile 384-well plates is needed when you dispense into and/or measure low-profile 384-well plates.

Can the Luminoskan Microplate Luminometer be upgraded for fluorometric applications?

Unfortunately, the Luminoskan Microplate Luminometer is a dedicated luminometer. It does not have a fluorometric option.

Can my Luminoskan Microplate Luminometer, Fluoroskan Microplate Fluorometer, or Fluoroskan FL Microplate Fluorometer and Luminometer perform bottom-reads for adherent cell assays?

Yes, it can. Follow the instructions for changing the measurement direction in the user manual: https://assets.thermofisher.com/TFS-Assets/BID/manuals/luminoskan-fluoroskan-fl-user-manual.pdf

Can I order the Luminoskan Microplate Luminometer, Fluoroskan Microplate Fluorometer, or Fluoroskan FL Microplate Fluorometer and Luminometer with injectors?

Yes, these readers can accommodate up to 3 injectors.

Can my Luminoskan Microplate Luminometer or Fluoroskan FL Microplate Fluorometer and Luminometer read different types of luminescence assays including kinetic, dual luminescence, flash, glow, BRET, BRET2, or NanoBRET assays?

Yes, Luminoskan Microplate Luminometer and Fluoroskan FL Microplate Fluorometer and Luminometer can read any type of luminescence assay. Some will require filters.

Is there a Windows 10 compatible software for my Fluoroskan Ascent Microplate Fluorometer, Fluoroskan Ascent FL Microplate Fluorometer and Luminometer, or Luminoskan Ascent Microplate Luminometer?

If your reader's serial number begins with 374-9 followed by more digits/letters, then you can order an upgrade kit that includes a USB converter kit and SkanIt Software (Cat. No. N17646). If the serial number is 374- followed immediately by any number other than 9, then you must retain an older Windows 7, 32-bit for PC and continue using Ascent 2.6 software, as your reader's firmware is not compatible with SkanIt Software.