Thermo Scientific™

CaptureSelect™ CH1-XL MiniChrom Column

Catalog number: 5943462005
Thermo Scientific™

CaptureSelect™ CH1-XL MiniChrom Column

Catalog number: 5943462005
Catalog Number
5943462005
Unit Size
5 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
59434620055 mL
-
59434620011 mL
-
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CaptureSelect CH1-XL MiniChrom columns are designed for bench-scale resin screening, process development, and sample preparation. These pre-packed columns are ready to connect and have the following features:

  • Dimensions of 1 mL (0.8 cm D x 2 cm L) and 5 mL (0.8 cm D x 10 cm L)
  • Direct connection to standard chromatography systems
  • Packed with CaptureSelect CH1-XL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

The 1 mL columns can be used for quick screenings of application feasibility and lab scale purification on a convenient and easy-to-use pre-packed column. The 10 cm bed height of the 5 mL columns allows initial process development on a bench scale.

The CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features of this affinity matrix include:

  • Platform approach for purification of high quality and correctly assembled human Fab fragments
  • No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
  • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
  • Caustic cleaning with up to 50 mM NaOH
  • Excellent scalability
  • No cross binding to bovine IgG, therefore presence of fetal bovine serum is not a problem

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect CH1-XL affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >15 mg/mL human Fab fragment, depending on flow rate, column height, and contact time. Note that humanized Fab fragments may have lower capacities.
  • Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0 - 4.5
  • Column dimensions: 1 mL (0.8 cm D x 2 cm L) and 5 mL (0.8 cm D x 10 cm L)
  • Flow characteristics: 75–150 cm/h (up to 2 bar)
  • Formulation buffer: 20%(v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Format
Chromatography Cartridge
Diameter (Metric)
0.8 cm
Flow Rate
75 to 150 cm/hr. (Up to 2 bar)
Length (Metric)
10 cm
Ligand Type
CH1-XL affinity ligand
Particle Size
65 ± 10 μm
Stationary Phase
CaptureSelect CH1-XL affinity ligand
Product Line
CaptureSelect
Binding Capacity
>15 mg/mL human Fab fragment
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Matrix
Agarose-based, epoxide-activated
Volume (Metric)
5 mL

Contents & Storage

Store in refrigerator (2°C to 8°C).

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