Thermo Scientific™

MabCaptureC™ MiniChrom Column

Catalog number: 5943662001
Thermo Scientific™

MabCaptureC™ MiniChrom Column

Catalog number: 5943662001
Catalog Number
5943662001
Unit Size
1 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
5 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
59436620011 mL
-
59436620055 mL
-
Product Overview
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MabCaptureC MiniChrom columns are designed for bench-scale resin screening, process development, and sample preparation. These pre-packed columns are ready to connect and have the following features:

  • Packed with MabCaptureC Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates
  • Direct connection to standard chromatography systems

The 1 mL columns can be used for quick screenings of application feasibility and lab scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5 mL column allows initial process development at a bench scale.

The pre-packed MabCaptureC Affinity Matrix contains engineered rProtein A that binds at the CH2-CH3 interface of the Fc-region of IgGs and shows cross binding to the VH3 domain of IgGs.

Features of MabCaptureC Affinity Matrix include:

  • High dynamic mAb binding capacity of > 50 g/L at 4.8 min residence time
  • Purification of mAbs and a subpopulation of scFv's and Fab fragments containing VH3 binding domains
  • Clean and sanitize with standard sodium hydroxide agents for chromatography products
  • Excellent scalability
  • Non-animal derived

Main resin characteristics:

  • Matrix: agarose-based, epoxide activated
  • Average particle size: 75 ± 10 μm
  • Ligand: recombinant C domain of Protein A
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >55 g IgG/L resin at 5 min residence time and 10% breakthrough; >75 g IgG/L resin at 10 min. residence time and 10% breakthrough
  • Elution conditions: 0.1 M glycine pH 3.0, or 20–100 mM sodium citrate or acetate, pH 3.0
  • Cleaning conditions: 0.2 M sodium hydroxide for 15 minutes every cycle
  • Column dimensions: 0.8 cm D x 2 cm L (1 mL); 0.8 cm D x 10 cm L (5 mL)
  • Operating pressure: ≤ 3 bar
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Bed Volume
1 mL
Binding Capacity
>55 g IgG/L resin (5 min. residence time and 10% breakthrough), >75 g IgG/L resin (10 min. residence time and 10% breakthrough)
Matrix
Agarose-based, Epoxide-activated
Diameter (Metric)
0.8 cm
Max. Operating Pressure
≤3 bar
Length (Metric)
2 cm
Particle Size
75 ±10 μm
Target
IgG mAbs, and a subpopulation of scFv and Fab containing VH3 domain
Ligand Type
Engineered rProtein A
Stationary Phase
Agarose bead

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