Invitrogen™

eBioscience™ Fixable Viability Dye eFluor™ 455UV

Catalog number: 65-0868-18
Invitrogen™

eBioscience™ Fixable Viability Dye eFluor™ 455UV

Catalog number: 65-0868-18
Catalog Number
65-0868-18
Unit Size
500 tests
Price (USD)
336.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 tests
Price (USD)
96.75
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
65-0868-18500 tests
336.00
Your Price:
***
65-0868-14100 tests
96.75
Your Price:
***
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Fixable Viability Dye eFluor™ 455UV is a viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. Fixable Viability Dyes may be used to label cells from all species.

Fixable Viability Dye eFluor™ 455UV can be excited by a UV laser line (∼350 nm) and has a peak emission of 455 nm that can be detected using a 450/50 band pass filter (equivalent to DAPI). Please make sure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 455UV is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture. Store at less than or equal to -70°C with dessicant. It may be freeze-thawed up to 20 times. Allow vial to equilibrate to room temperature before opening.

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Blue
Detection Method
Fluorescent
Emission
350⁄455
Product Line
eBioscience™
Quantity
500 tests
For Use With (Equipment)
Flow Cytometer

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