McCoy's 5A Medium (Park and Terasaki modification)

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. Park and Terasaki modified McCoy’s medium with modified levels of glycine, L-glutamic acid, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine, plus the addition of antibiotics and HEPES.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Gibco™ McCoy's 5A (Park and Terasaki modification) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (Park and Terasaki modification) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.