The CaptureSelect VIIISelect Leakage ELISA is designed to detect possible leached ligand of B domain-depleted recombinant Factor VIII purified using VIIISelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the VIIISelect affinity ligand can leach off of the chromatography support and co-elute with human Factor VIII. This ELISA provides typical sensitivity and accurate quantitation of possible leached ligand greater than or equal to 1 ng/mL. The CaptureSelect VIIISelect Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
For Research Use Only. Not for use in diagnostic procedures.