The CaptureSelect™ hCG Ligand Leakage ELISA detects contamination of CaptureSelect hCG affinity ligand in human chorionic gonadotropin (hCG) purified using CaptureSelect hCG Affinity Matrix. In this affinity matrix the hCG affinity ligand, a 13 kD recombinant protein, is coupled by multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This ELISA enables the detection of affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The hCG Ligand Leakage ELISA kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
For Research Use Only. Not for use in diagnostic procedures.