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View additional product information for GeneArt™ Synthetic Gene (Complexity Service fee) - FAQs (817017DE)
21 product FAQs found
TALs are transcription activator-like effector proteins that are naturally occurring transcriptional activators. GeneArt Precision TALs allow researchers to determine the exact DNA sequence they would like to have their functionality delivered to and have specific TAL genes built to perform the function.
Please use the following link (http://www.thermofisher.com/us/en/home/life-science/synthetic-biology/synthetic-biology-services.html) to explore our synthetic biology services which include: vector construction, cDNA library construction, protein expression services, directed evolution services, plasmid services, and cell line & protein services.
Yes, we will optimize the protein based on the species you choose to provide you with the optimized gene sequence for your studies.
No, your custom vector has been banked and sequenced and is available to you in your individual short list “My Portal Vectors” as soon as they have been ordered/used once.
Partial deliveries can occur when upon reaching the estimated completion date not all order items have been finalized. Partial shipments of incomplete orders are initiated on a weekly basis. Separate shipping of items does not incur an extra cost.
Generally, we ship immediately upon completion of the project item with the longest estimated turnaround time. In rare cases, completely finalized projects are not shipped until the estimated completion date has been reached. Please keep in mind, if you order gene synthesis and subcloning as a process, you will most likely receive the gene synthesis in a Geneart vector before you receive your gene in your subcloned vector.
You can download your optimized sequence in FASTA format. It can be read in a txt file/format. The FASTA file will give a simple description of the gene, and then give a non-annotated version of the sequence. You can align this file with their original sequence using a free online tool, or if you have Vector NTI software, you can use this to align the files.
You typically would receive the following with your order: vector map, alignment maps, sequencing traces. Extended documentation can include:
1. Data storage certificate - guaranteed storage of all production specific data
2. Material documentation - includes all information on all used chemicals including providers and catalog numbers
3. GLP source documentation - includes the granularity of the material documentation to lot number level of all used reagents
TSE stands for Transmissible Spongiform Encephalopathy; ie media is based on soy for TSE-free plasmid preps.
Yes. There are different compliance regulations/rules for synthesis, including the use of different labs. With Biosafety 2, it takes a longer time to synthesize genes, therefore, these projects may be more expensive. Please note, regardless of your selection, Thermo Fisher Scientific Corporation and/or its affiliates will perform a biosafety evaluation. In the case that you select Biosafety Level 1 but our evaluation requires the DNA to be a Biosafety Level 2, you will be sent a modified quotation according to the Biosafety Level 2 requirements for review.
Please provide 5-10µg of vector DNA for your GeneArt custom service. We prefer either dissolved in TE or water, or as a pellet.
You will receive both vectors - your gene in the basic cloning vector, and your gene in the subcloned vector of your choice.
Yes, we keep a sample of every GeneArt-synthesized product.
GeneArt synthesis adds standard restriction sites for automated cloning into GeneArt standard vectors in addition to your selected restriction sites.
No, and this cannot be requested. You would need to subclone into a vector of your choice. The only thing you can request as part of the “Geneart vector” is the antibiotic resistance (for a small markup fee).
We do not offer free choices of restriction sites in our standard production vectors. For our production process, restriction enzymes are added that generally eliminate most of the standard vectors' MCS. The reason why the MCS contains more sites is to allow for fallback scenarios in case certain sites cannot be used due to insert sequence intrinsic restrictions.
Production time is based on gene length and choice of shipment speed. Please see this link (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneart-standard-and-xxl-genes.html) for the differences between our shipping conditions.
GeneArt Strings DNA Fragments are uncloned double-stranded DNA fragments that are limited to length of 1,000 bp. GeneArt Gene Synthesis products are double-stranded DNA fragments that have been cloned into a standard GeneArt vector (pMx). Please note, a sequence may be too complex to be produced as a Strings DNA Fragment due to repetitions or high GC content. In this case, our scientists will recommend that you order your gene via gene synthesis. You could try optimizing your sequence through the GeneOptimizer algorithm which, in some cases, could optimize the sequence and allow the DNA Fragments to be ordered.
GeneObserver shows the manufacturing status of your web and non-web orders, as well as the completed date so that you can track your gene synthesis project.
Fath et al. (2011) A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression. PLoS ONE 6(3): e17doi:10.1371/journal.pone.0017596
A project can have multiple processes, and therefore, can be ordered as single project. In this way shipping/handling are paid only once.
Single variants and variant bundles (more than one variant process) are variants of a master gene. The variant criteria are as follows:
Variants may contain up to four of the following modifications:
- 5'/3' deletion of any length with additional 52 nt of new sequence on each side
- 5'/3' modification of a maximum of 52 nt on each side
- 5'/3' extension of a maximum of 52 nt on each side
- Internal modifications of up to 40 nt (a maximum of 3 of these internal modification blocks are allowed)
- Internal deletions of any length
- Modifications (whether internal or 5´/3´) must be separated by at least 100 bp.
If a gene falls outside of these criteria, it must be created as a separate gene synthesis instead of as a variant.