GeneArt™ Mutagenesis, <1 kb - FAQs

Q: What are the gene synthesis requirements for Express cloning?

Genes qualifying for Express cloning must be <4 kb and not complex (optimization of your sequence can reduce complexity).

Q: What vectors are available for Express cloning?

The following vectors are currently available: - pcDNA 3.1(+)vector - pcDNA3.3-TOPO vector - pcDNA3.4-TOPO vector - pFastBac1 vector - pET100/D-TOPO vector - pET151/D-TOPO vector - pRSET A vector - pYes2.1V5-His TOPO vector

Q: What will I receive if I use your GeneArt Express Cloning Service?

You should receive your synthesized gene in the selected expression vector. Please note, no additional copy in a pMx cloning vector will be provided as with traditional GeneArt Cloning Services.

Q: What is the GeneArt Express Cloning Service?

Using an alternative production procedure with pre-prepared expression vectors, our GeneArt custom service team can clone your synthesized genes directly into selected Thermo Fisher Scientific expression plasmids, saving 4-5 business days compared to the standard workflow with cloning into a pMx series vector first. Please visit this page (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-plasmid-services/geneart-subcloning-service.html) for the differences between Express cloning and Classical cloning.

Q: I would like to order several variants of the same gene synthesis gene project. Can I do this?

Yes, you can set up mutagenesis bundles as gene variants to order multiple gene variant sequences.

View additional product information for GeneArt™ Mutagenesis, <1 kb - FAQs (830105DE)

5 product FAQs found

What are the gene synthesis requirements for Express cloning?

Genes qualifying for Express cloning must be <4 kb and not complex (optimization of your sequence can reduce complexity).

What vectors are available for Express cloning?

The following vectors are currently available:

- pcDNA 3.1(+)vector
- pcDNA3.3-TOPO vector
- pcDNA3.4-TOPO vector
- pFastBac1 vector
- pET100/D-TOPO vector
- pET151/D-TOPO vector
- pRSET A vector
- pYes2.1V5-His TOPO vector

What will I receive if I use your GeneArt Express Cloning Service?

You should receive your synthesized gene in the selected expression vector. Please note, no additional copy in a pMx cloning vector will be provided as with traditional GeneArt Cloning Services.

What is the GeneArt Express Cloning Service?

Using an alternative production procedure with pre-prepared expression vectors, our GeneArt custom service team can clone your synthesized genes directly into selected Thermo Fisher Scientific expression plasmids, saving 4-5 business days compared to the standard workflow with cloning into a pMx series vector first. Please visit this page (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-plasmid-services/geneart-subcloning-service.html) for the differences between Express cloning and Classical cloning.

I would like to order several variants of the same gene synthesis gene project. Can I do this?

Yes, you can set up mutagenesis bundles as gene variants to order multiple gene variant sequences.