See our new Abundant Protein Depletion Columns- High-Select&trade HSA/Immunoglobulin Depletion Mini Spin Columns- that remove >95% of albumin and >95% of all major subclasses of immunoglobulins in only 10 minutes.
Thermo Scientific™

Pierce™ Top 2 Abundant Protein Depletion Spin Columns

Catalog number:  85161

 Related applications:

Protein Mass Spectrometry Analysis

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Thermo Scientific Pierce Abundant Protein Depletion Spin Columns are optimized to decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or 1D and 2D gel electrophoresis.

Features of Top 2 Abundant Protein Depletion Spin Columns:

Optimized—resin in spin columns is scaled and optimized for treating 10 µL (600 µg) of human plasma samples for mass spectrometry and/or 1D and 2D electrophoresis
Efficient—kits remove >90% of IgG and >95% of albumin, plus other abundant proteins (Top 12)
Fast—process samples in 40 to 60 minutes (depending on resin)
Economical—cost effective spin columns are priced for single use
Reduce variability—One time use prevents abundant protein carryover and experimental variability
Two resin options—choose the system appropriate for your need: 2 or 12 protein depletion columns

The Pierce Top 2 Abundant Protein Depletion Spin Columns use highly specific immobilized anti-HSA and anti-IgG antibodies to remove human serum albumin (HSA) and all major subclasses of gamma globulin (IgG) from serum, plasma or spinal fluids. The resins are provided in an economical and convenient spin column specifically designed one step processing and for single use.

Analysis of human fluids is often complicated by the presence of high concentrations of albumin and IgG that can make up more than 70% of total serum protein. Removal of these proteins is often essential for the study of low-abundant proteins. The Pierce Top 2 Protein Depletion Columns can deplete greater than 99% of HSA and 90% of IgG. The Pierce Top 12 Protein Depletion Columns can deplete greater than 95% of the twelve most abundant proteins in serum. Samples are loaded in a pre-filled convenient disposable spin format and can be processed in 40 to 60 minutes. The depletion of highly abundant proteins enables the detection of low abundant proteins in samples and subsequent identification by mass spectrometry or 1D and 2D gel electrophoresis.

Related Products
Pierce™ Top 12 Abundant Protein Depletion Spin Columns
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Mass Spectrometry
Final Product: Protein
For Use With (Equipment): Mass Spectrometer, Microcentrifuge
Product Line: Pierce™
Product Size: 6 columns
Starting Material: Plasma, Serum
Workflow Step: Sample Clean Up, Sample Enrichment

Contents & storage

Store at 4°C—do not freeze


Manuals & protocols