Pierce™ Detergent Removal Resin

Catalog number: 87780

Thermo Scientific™  Related applications: Protein Purification & Isolation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Thermo Scientific Pierce Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

Features of Detergent Removal Resin:

• Fast and efficient protocol
• Removes greater than 95% of detergents with high protein recovery
• Helps improves mass spectrometry peptide coverage
• Best for processing samples with proteins or peptides at greater than 100 µg/mL
(for low-concentration samples, consider our HiPPR Detergent Removal Products)

The Pierce Detergent Removal Resin specifically binds a wide variety of detergents and surfactants that are commonly used in protein extraction and biological sample preparation and provide an especially convenient and rapid format for treating protein and peptide solutions to remove interfering detergents before downstream analysis by mass spectrometry and other techniques.

Detergent removal from peptide samples is a challenge, especially for MS analysis in which even low detergent concentrations contaminate instruments and interfere with column binding, elution and ionization. Experiments demonstrate that Pierce Detergent Removal Resin effectively removes a variety of detergents from BSA and HeLa cell lysate tryptic digests, enabling improved LC-MS/MS and MALDI-MS analysis. After processing samples, the high baseline caused by detergents is reduced or eliminated. Analysis of the digested HeLa cell lysates by LC-MS/MS resulted in an approximate four-fold increase of identified peptides in the treated sample compared to the detergent-contaminated sample and equivalent numbers of peptides compared to a control sample, indicating minimal losses of peptides.

We recommend Pierce Detergent Removal Resin for samples having protein or peptide concentrations greater than 100 µg/mL. Use the HiPPR Detergent Removal Resin (where "HiPPR" denotes High Protein and Peptide Recovery) for sample concentrations of 1 to 100 µg/mL.

Related Products
Pierce™ Detergent Removal Spin Column, 4 mL
Pierce™ Detergent Removal Spin Column, 2 mL
Pierce™ Detergent Removal Spin Column, 125 µL
HiPPR™ Detergent Removal Spin Column Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Bottle(s)
Column Type: Affinity, Proprietary Resin
Product Size: 10 mL
Immobilized Phase: Proprietary Ligand

Contents & storage

Upon receipt store at 4°C. The resin is stored in 0.15 M NaCl, 0.05% sodium azide.

Documents

Manuals & protocols

Recommended products