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Mouse IgG (Total) Uncoated ELISA Kit - FAQs

Mouse IgG (Total) Uncoated ELISA Kit - FAQs

View additional product information for Mouse IgG (Total) Uncoated ELISA Kit - FAQs (88-50400-77, 88-50400-88)

6 product FAQs found

I ordered your Mouse IgG Total Uncoated ELISA kit (Cat. No. 88-50400-88) and found there some crystal precipitation in the 20X Assay Buffer A. Is it still usable?

This buffer is primarily PBS, Tween and BSA.
It is completely normal to see crystals/precipitate in BSA solutions and they should be dissolved completely at 37 degrees C (in less than 30 minutes). Gentle agitation may help dissolve the crystals faster. It is better do not increase the temperature more that 37-42 degrees C, when dissolving, as BSA can denature (>60 degrees C).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What type of plates are included in the Ready-SET-Go! ELISA sets?

Corning Costar 96-well plates are included with Ready-SET-Go! ELISA catalog numbers ending in suffixes -22, -86 and -76. These solid 96-well plates are optional with the 10 and 20 plate kit sizes.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What is the sensitivity of your Ready-Set-Go! ELISA Sets?

The sensitivity ranges of our Ready-SET-Go! ELISA sets vary depending on the cytokine/protein being measured. Please refer to the product datasheet for set specific information.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What is the expiration date for your Ready-Set-Go! ELISA Sets?

The majority of our Ready-Set-Go! ELISA Sets are guaranteed for one year from the date of shipment. However, we do have several sets that are guaranteed for 6 months upon date of receipt. Please refer to the Certificate of Analysis for shelf-life information.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What are the storage conditions for your Ready-Set-Go! ELISA Sets?

All of the reagents are to be stored at 4 degrees C upon receipt, except for the recombinant standards. The recombinant standards MUST be stored at or below -80 degrees C to ensure stability and performance.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do Ready-SET-Go! ELISA Sets contain?

Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
10X Coating Buffer
5X ELISA/ELISPOT Diluent
Detection enzyme: pre-titrated Avidin-HRP
Substrate Solution: 1X TMB (Tetramethylbenzidine) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.