Invitrogen™

eBioscience™ Annexin V Apoptosis Detection Kits

Catalog number: 88-8008-74
Invitrogen™

eBioscience™ Annexin V Apoptosis Detection Kits

Catalog number: 88-8008-74
Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.
 
Catalog Number
88-8008-74
Unit Size
200 tests
Conjugate
PCP-eFluor 710
Excitation/Emission
Price (USD)
428.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V Apoptosis Detection Kit PerCP-eFluor 710
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V PerCP-eFluor 710, 1 vial (1 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Shipping ConditionWet Ice
ConjugatePCP-eFluor 710
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
Catalog Number
Unit Size
200 tests
Conjugate
FITC, Propidium Iodide
Excitation/Emission
488, 535/520, 617
Price (USD)
489.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V Apoptosis Detection Kit FITC
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V-FITC conjugate, 1 vial (1 mL)
• Propidium Iodide Staining Solution, 2 vials (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Product LineeBioscience
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
Catalog Number
Unit Size
50 tests
Conjugate
eFluor 450, 7-AAD
Excitation/Emission
405, 546/450, 647
Price (USD)
224.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionAnnexin V Apoptosis Detection Kit eFluor 450
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V eFluor 450, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission405, 546/450, 647
Shipping ConditionWet Ice
Product LineeBioscience
ConjugateeFluor 450, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size50 tests
Catalog Number
Unit Size
200 tests
Conjugate
eFluor 450, 7-AAD
Excitation/Emission
405, 546/450, 647
Price (USD)
398.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V Apoptosis Detection Kit eFluor 450
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V eFluor 450, 1 vial (1 mL)
• 7-AAD Viability Staining Solution, 2 vials (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission405, 546/450, 647
Shipping ConditionWet Ice
ConjugateeFluor 450, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
Catalog Number
Unit Size
200 tests
Conjugate
APC, Propidium Iodide
Excitation/Emission
650, 535/660, 617
Price (USD)
542.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V Apoptosis Detection Kit APC
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V APC, 1 vial (1 mL)
• Propidium Iodide Staining Solution, 2 vials (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission650, 535/660, 617
Shipping ConditionWet Ice
ConjugateAPC, Propidium Iodide
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
Catalog Number
Unit Size
50 tests
Conjugate
PCP-eFluor 710
Excitation/Emission
Price (USD)
234.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V Apoptosis Detection Kit PerCP-eFluor 710
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V PerCP-eFluor 710, 1 vial (0.25 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Shipping ConditionWet Ice
ConjugatePCP-eFluor 710
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size50 tests
Catalog Number
BMS500FI-100
also known as BMS500FI/100
Unit Size
100 tests
Conjugate
FITC, Propidium Iodide
Excitation/Emission
488, 535/520, 617
Price (USD)
276.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V-FITC Apoptosis Detection Kit
Kit Contents• Binding Buffer (4X), 1 bottle (50 mL)
• rh Annexin V-FITC, 1 vial (0.5 mL)
• Propidium Iodide (20 µg/mL), 1 vial (1.8 mL)
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Shipping ConditionWet Ice
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Flow Cytometer
Unit Size100 tests
Catalog Number
BMS500FI-20
also known as BMS500FI/20
Unit Size
20 tests
Conjugate
FITC, Propidium Iodide
Excitation/Emission
488, 535/520, 617
Price (USD)
132.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V-FITC Apoptosis Detection Kit
Kit Contents• Binding Buffer (4X), 1 bottle (50 mL)
• rh Annexin V-FITC, 1 vial (0.5 mL)
• Propidium Iodide (20 µg/mL), 1 vial (1.8 mL)
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Shipping ConditionWet Ice
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Flow Cytometer
Unit Size20 tests
Catalog Number
BMS500FI-300
also known as BMS500FI/300
Unit Size
300 tests
Conjugate
FITC, Propidium Iodide
Excitation/Emission
488, 535/520, 617
Price (USD)
459.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V-FITC Apoptosis Detection Kit
Kit ContentsContains:
• Binding Buffer (4X), 1 bottle (50 mL)
• rh Annexin V-FITC, 1 vial (1.5 mL)
• Propidium Iodide (20 µg/mL), 2 vials (1.8 mL)
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Shipping ConditionWet Ice
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Flow Cytometer
Unit Size300 tests
Catalog Number
Unit Size
50 tests
Conjugate
PE, 7-AAD
Excitation/Emission
Price (USD)
158.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptioneBioscience Annexin V Apoptosis Detection Kit PE
Kit ContentsContains:
• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V PE, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Shipping ConditionWet Ice
ConjugatePE, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size50 tests
Showing 10 of 18Show more
Catalog NumberSpecificationsUnit SizeConjugateExcitation/EmissionPrice (USD)AvailabilityQuantity
88-8008-74Full specifications
200 testsPCP-eFluor 710
428.00
Your Price:
***
DescriptioneBioscience Annexin V Apoptosis Detection Kit PerCP-eFluor 710
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V PerCP-eFluor 710, 1 vial (1 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Shipping ConditionWet Ice
ConjugatePCP-eFluor 710
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
88-8005-74Full specifications
200 testsFITC, Propidium Iodide488, 535/520, 617
489.00
Your Price:
***
DescriptioneBioscience Annexin V Apoptosis Detection Kit FITC
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V-FITC conjugate, 1 vial (1 mL)
• Propidium Iodide Staining Solution, 2 vials (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Product LineeBioscience
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
88-8006-72Full specifications
50 testseFluor 450, 7-AAD405, 546/450, 647
224.00
Your Price:
***
DescriptionAnnexin V Apoptosis Detection Kit eFluor 450
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V eFluor 450, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission405, 546/450, 647
Shipping ConditionWet Ice
Product LineeBioscience
ConjugateeFluor 450, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size50 tests
88-8006-74Full specifications
200 testseFluor 450, 7-AAD405, 546/450, 647
398.00
Your Price:
***
DescriptioneBioscience Annexin V Apoptosis Detection Kit eFluor 450
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V eFluor 450, 1 vial (1 mL)
• 7-AAD Viability Staining Solution, 2 vials (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission405, 546/450, 647
Shipping ConditionWet Ice
ConjugateeFluor 450, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
88-8007-74Full specifications
200 testsAPC, Propidium Iodide650, 535/660, 617
542.00
Your Price:
***
DescriptioneBioscience Annexin V Apoptosis Detection Kit APC
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (100 mL)
• Annexin V APC, 1 vial (1 mL)
• Propidium Iodide Staining Solution, 2 vials (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission650, 535/660, 617
Shipping ConditionWet Ice
ConjugateAPC, Propidium Iodide
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size200 tests
88-8008-72Full specifications
50 testsPCP-eFluor 710
234.00
Your Price:
***
DescriptioneBioscience Annexin V Apoptosis Detection Kit PerCP-eFluor 710
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V PerCP-eFluor 710, 1 vial (0.25 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Shipping ConditionWet Ice
ConjugatePCP-eFluor 710
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size50 tests
BMS500FI-100
also known as BMS500FI/100
Full specifications
100 testsFITC, Propidium Iodide488, 535/520, 617
276.00
Your Price:
***
DescriptioneBioscience Annexin V-FITC Apoptosis Detection Kit
Kit Contents• Binding Buffer (4X), 1 bottle (50 mL)
• rh Annexin V-FITC, 1 vial (0.5 mL)
• Propidium Iodide (20 µg/mL), 1 vial (1.8 mL)
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Shipping ConditionWet Ice
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Flow Cytometer
Unit Size100 tests
BMS500FI-20
also known as BMS500FI/20
Full specifications
20 testsFITC, Propidium Iodide488, 535/520, 617
132.00
Your Price:
***
DescriptioneBioscience Annexin V-FITC Apoptosis Detection Kit
Kit Contents• Binding Buffer (4X), 1 bottle (50 mL)
• rh Annexin V-FITC, 1 vial (0.5 mL)
• Propidium Iodide (20 µg/mL), 1 vial (1.8 mL)
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Shipping ConditionWet Ice
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Flow Cytometer
Unit Size20 tests
BMS500FI-300
also known as BMS500FI/300
Full specifications
300 testsFITC, Propidium Iodide488, 535/520, 617
459.00
Your Price:
***
DescriptioneBioscience Annexin V-FITC Apoptosis Detection Kit
Kit ContentsContains:
• Binding Buffer (4X), 1 bottle (50 mL)
• rh Annexin V-FITC, 1 vial (1.5 mL)
• Propidium Iodide (20 µg/mL), 2 vials (1.8 mL)
Product TypeApoptosis Detection Kit
Excitation/Emission488, 535/520, 617
Shipping ConditionWet Ice
ConjugateFITC, Propidium Iodide
For Use With (Equipment)Flow Cytometer
Unit Size300 tests
88-8102-72Full specifications
50 testsPE, 7-AAD
158.00
Your Price:
***
DescriptioneBioscience Annexin V Apoptosis Detection Kit PE
Kit ContentsContains:
• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V PE, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Shipping ConditionWet Ice
ConjugatePE, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit Size50 tests
Showing 10 of 18Show more
Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

Contents & Storage

2°C to 8°C. Avoid temperature fluctuations.

Figures