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Human IL-8 Uncoated ELISA Kit - FAQs

Human IL-8 Uncoated ELISA Kit - FAQs

View additional product information for Human IL-8 Uncoated ELISA Kit - FAQs (88-8086-77, 88-8086-88)

5 product FAQs found

With the IL-8 Human Uncoated ELISA Kit (Cat. No. 88-8086-88), can I store the coated ELISA plates for later use?

Yes, the coated ELISA plates can be stored for a maximum period of 2-3 days in the capture antibody solution (100 µL/well of capture antibody in Coating Buffer). We recommend sealing the plates with Parafilm material and keeping them on a level surface at 4 degrees C. We do not recommend drying the plates for long-term storage as our assays are not optimized for these conditions.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What are the critical parameters for successful ELISPOT assay?

A: It is critically important to use a high-affinity binding PVDF membrane plate, rather than regular ELISA plate or nitrocellulose membrane plate. Additionally, highest affinity antibodies for capture work best in this assay. Empirical optimization of conditions such as cell density, stimulus/mitogen, and kinetics is necessary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I recently bought one of your Ready-Set-Go! ELISA Sets, can I use the antibody pairs to run an ELISPOT?

We do not recommend the use of our ELISA kits for the ELISPOT application. We cannot guarantee the successful use of our ELISA kits in an ELISPOT application.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Do your Ebioscience antibody pairs work in ELISPOT as well?

Most, but not all, of our ELISA antibody pairs work for ELISPOT. We continue to evaluate and optimize our anti-human and anti-mouse cytokine antibody pairs for ELISPOT applications. Please see our protocols page (www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/protocols) for a detailed protocol and a reference table with the list of antibody pairs that can be used in ELISPOT.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the components of the Ready-Set-Go! ELISPOT Sets?

Capture Antibody: Pre-titrated, Functional Grade (low endotoxin) purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
10X Sterile Azide-Free Coating Buffer
5X ELISA/ELISPOT Diluent
Detection enzyme: pre-titrated Avidin HRP
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and enzyme

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.