pEF1α Tluc16-DD Vector for Luciferase Assays

Catalog number: 88241

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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The Thermo Scientific™ pEF1α Tluc16 Vector encodes the smallest known luciferase, TurboLuc™16 (Tluc16) luciferase, as a reporter under a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells.

• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Truncated EF1α promoter for constitutive Tluc16 luciferase expression

Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: EF-1a
Key Function: Reporter Assays
Reporter: TurboLuc™16 Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Product Line: Pierce™
Product Size: 10 µg

Contents & storage

Store in freezer (-5 to -30°C).


Manuals & protocols