pCRE Tluc16-DD Vector for Luciferase Assays

Catalog number: 88247

Thermo Scientific™  Related applications: Protein Assays and Analysis | Protein Expression

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Description

The pCRE Tluc16-DD Vector is a transcriptional reporter vector designed to monitor the activation of cAMP-binding protein (CREB) and cAMP-mediated signal transduction pathways in mammalian cells. The vector encodes the smallest known luciferase, TurboLuc™16 (Tluc16) luciferase, as the reporter under the control of a combination of an optimized minimal core promoter and 5 tandem repeats of the c-AMP response element (CRE).

• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
• Monitor the activation of cAMP-binding protein (CREB) and cAMP-mediated signal transduction pathways in mammalian cells

Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression.

The pCRE Tluc16-DD luciferase expression vector also contains dual-destabilization (DD) technology that reduces accumulation of the Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the cAMP response elements to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: minP-CRE
Key Function: Reporter Assays
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 10 µg

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols