Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.
Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO), gamma-irradiated:
• Easy-loading—pipette-accessible for easy sample loading and retrieval • Self-floating—integrated air chambers eliminate the need for float buoys • Sturdy construction—ensures the highest possible sample integrity and protection • Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery • Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting • Gamma-irradiated—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination • Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
10K Dialysis Membrane Specifications • Composition: Regenerated cellulose synthesized by the Viscose method • Thickness: 0.9 to 1.2mil (22.5 to 30 µM) • Hydration Required Before Use: 30 seconds for small-volume samples • Glycerol Content: 21% • Sulfur Content: 0.05% • Heavy Metals Content: Trace
The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.
Gamma-irradiated (Sterile) Dialysis Cassettes: Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.