Thermo Scientific™

Powdered DMEM Medium for SILAC

Catalog number: 88425
Thermo Scientific™

Powdered DMEM Medium for SILAC

Catalog number: 88425
Catalog Number
88425
Unit Size
135 g
Price (USD)
295.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
88425135 g
295.00
Your Price:
***
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Thermo Scientific Powdered DMEM Medium for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of Powdered DMEM Medium for SILAC:

Flexible—powdered medium deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
High quality—medium is sterile, endotoxin-free and cell culture-compatible

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC medium supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Specifications

Quantity
135 g
Shipping Condition
Wet Ice
Final Product Type
Protein
Starting Material
Cells
Workflow Step
Protein Labeling
For Use With (Equipment)
Mass Spectrometer
Product Type
Media

Contents & Storage

135 g of powdered medium to make 10 L of medium.

Store in refrigerator (2 to 8°C).

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