Pierce™ Protein Concentrators PES, 50K MWCO, 20–100 mL, 4 Concentrators - FAQs

The dimensions of the 20-100 mL capacity concentrators (Pierce Protein Concentrators PES, 3K or 5K MWCO, 0.5–100 mL (Cat. No. 88534), Pierce Protein Concentrators PES, 10K MWCO, 0.5–100 mL (Cat. No. 88535), Pierce Protein Concentrators PES, 30K MWCO (Cat No. 88536), Pierce Protein Concentrators PES, 50K MWCO, 0.5–100 mL (Cat. No. 88542), and Pierce Protein Concentrators PES, 100K MWCO, 0.5–100 mL (Cat No. 88537)) are 61 mm outer diameter and 124 mm height. These are similar to Nalgene and Nunc 250 mL bottles. Adapters to the Nunc 250 mL bottles will fit to the 20-100 mL capacity concentrators.

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We do not recommend autoclaving the PES protein concentrators. High temperatures will significantly increase the membrane molecular weight cutoff (MWCO). To sterilize, we recommend using a 70% ethanol solution.

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Yes, the PES membrane is compatible with desalting and buffer exchange. Protocols for concentrating, desalting, and buffer exchange only are provided in the product manuals.

The protein concentrator columns have an upper compartment that holds the sample, and a lower compartment. The two compartments are separated by a polyethersulfone (PES) semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The protein concentrator cassettes also contain a PES membrane and a unique channel design that ensures high optimal flux with no optimization effort. Unlike other systems, the fluid is passed parallel to the filter, rather than being pushed through a membrane perpendicularly, which can clog the filter. For maximum protein recovery in both columns and cassettes, samples should have a molecular weight two-fold greater than the molecular weight cutoff (MWCO) of the concentrator membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Pierce polyethersulfone (PES) protein concentrators are offered in six distinct MWCOs of 3K, 5k, 10K, 30K, 50K, and 100K, and the cassettes are offered in five membrane MWCOs of 3K, 10K, 30K, 50K, and 100K. The columns can process four different sample volumes ranges of 100-500 µL, 2-6 mL, 5-20 mL, and 20-100 mL, while the cassettes can process 0.5-6 L. Please see our selection chart and video found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/protein-concentrators.html).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.