1-Step Human Coupled IVT Kit - DNA, 8 Reaction Kit - FAQs

View additional product information for 1-Step Human Coupled IVT Kit - DNA - FAQs (88882, 88881)

4 product FAQs found

What lysate systems can be used for in vitro protein expression?

E. coli, rabbit reticulocyte lysate (RRL), and HeLa cell lysate can all be used for in vitro translation. Our Expressway system utilizes E. coli. In general, RRL efficiently translates proteins greater than 30 kDa. The 1-Step Human In Vitro Protein Expression Kits are ideal for expressing human proteins.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can I use 1-Step Human Coupled IVT Kit - DNA (Cat. No. 88881, 88882) with mRNA?

This page outlines a protocols for in vitro protein expression using mRNA templates.

When comparing 1-Step Human High-Yield Mini IVT Kit with 1-Step Human Coupled IVT Kit - DNA, is one faster than the other?

No, both 1-Step Human High-Yield Mini IVT Kit and 1-Step Human Coupled IVT Kit - DNA can be run overnight (depends upon the gene).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Do both 1-Step Human High-Yield Mini IVT Kit and 1-Step Human Coupled IVT Kit - DNA provide the same purity of protein?

Yes, both 1-Step Human High-Yield Mini IVT Kit and 1-Step Human Coupled IVT Kit - DNA provide the same purity of protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.