GlycoLink™ IP Kit - FAQs

GlycoLink gel is hydrazide-activated crosslinked beaded agarose, and it is useful for coupling glycoproteins via aldehydes formed from their sugars by sodium meta-periodate oxidation. Reaction of aldehydes with hydrazide- activated resin is catalyzed by aniline resulting in >90% coupling in 4 hours or less. CarboxyLink gel on the other hand is crosslinked beaded agarose activated with diaminodipropylamine (DADPA) and is useful for immobilizing carboxyl- containing biomolecules after EDC activation. Actually, both resins can be used with EDC to couple ligands via carboxylic acids.

Note: Both immobilization chemistries are available on UltraLink Support as UltraLink Hydrazide and UltraLink DADPA respectively.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Yes, Chicken IgY can be immobilized using GlycoLink Gel, due to the high level of glycosylation of the IgY.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

No. Monoclonal antibodies are typically under-glycosylated, and coupling efficiency to CarboLink Gel may be low. However, many monoclonal antibodies can be immobilized using GlycoLink Gel. Try the Pierce Glycoprotein Carbohydrate Estimation Kit (Cat. No. 23260) to determine if your monoclonal antibody is glycosylated.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The benefits include high-capacity immobilization of 1-10 mg of oxidized antibody or other glycoprotein per milliliter of resin. Immobilization is fast (in as few as 30 mins) and can achieve at least 90% coupling of most glycoproteins in less than 4 hours. The hydrazide-activated UltraLink resin conjugates only to purified glycoproteins whose sugar groups have been gently oxidized with periodate.

No stabilization step is required when using this resin, and antibody function is preserved as IgGs are immobilized via the Fc region, keeping both antigen binding sites available for capturing targets.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.