P-PER™ Plant Protein Extraction Reagent

Catalog number: 89803

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

The Thermo Scientific P-PER Plant Protein Extraction Kit effectively extracts protein from all kinds of dry and fresh plant tissue without liquid nitrogen.

Features of the P-PER Plant Protein Extraction Kit:

Versatile—works with multiple plant organs (leaf, stem, root, seed and flowers); multiple plant species (Arabidopsis, tobacco, maize, soybeans, peas, spinach and other plant tissues); and fresh, frozen and dehydrated plant tissues
Convenient—requires no liquid nitrogen/freeze-grinding, Dounce homogenization, blade-shearing or glass-bead agitation for cell disruption; however, the P-PER Kit is compatible with these alternative mechanical aids
Compatible—downstream applications include 1-D and 2-D gel electrophoresis, Western blotting, activity assays, and protein affinity purifications
Quantifiable—P-PER Kit extracts can be quantified using the BCA Protein Assay Kit, Reducing Agent Compatible (Part No. 23250)
Ready-to-use—protein extract does not require filtration through cheesecloth or Miracloth, unlike homebrews
Fast—perform plant cell lysis and protein extraction in 10 minutes
Recovers active protein—assays show extracted proteins are functional

The P-PER Kit includes an organic lysing reagent and two aqueous reagents that effectively lyse plant cells and solubilize protein without harsh mechanical lysis aids, such as a mortar and pestle. Plant protein extracts are prepared in just 10 minutes, and protein yields exceed conventional extraction methods (e.g., freeze/grinding in liquid nitrogen) and other commercially available plant protein extraction reagents. The resulting protein extracts are compatible with a variety of downstream applications including the Thermo Scientific BCA Protein Assay Kit - Reducing Agent Compatible (Part No. 23250), 1D and 2D gel electrophoresis, immunoprecipitation, Western blotting, activity assays and protein purification.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Tissue Extraction Buffer, Cell Lysis Buffer, Detergent Solution
Form: Kit, Liquid
Product Size: 40 mL

Contents & storage

Upon receipt store at room temperature.

Documents

Manuals & protocols