Lysozyme - FAQs

Lysozyme powder can be dissolved in 0.1 M KH₂PO₄ (pH 6.24) to the desired concentration - up to 5 mg/mL. Once the solution has been prepared, it can be stored at 4 degrees C for up to 5-6 weeks, or at room temperature for up to 6-7 days, without affecting performance.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

The B-PER Reagent solution contains a proprietary, mild, non-ionic detergent in 20 mM Tris-HCl, pH 7.5. It effectively disrupts cells and solubilizes native or recombinant proteins without denaturation. The reagent creates holes in the cell membrane that will leak out cytosolic proteins. The sample may become very viscous when the bacterial chromosome is released. We recommend adding DNAse I (Cat. No. 90083) to the reagent to reduce viscosity. For better lysis efficiency and if there are inclusion bodies, we recommend adding Lysozyme (Cat. No. 90082) to the reagent. Alternatively, you may purchase the B-PER Bacterial Protein Extraction Reagent with Enzymes Kit (Cat. No. 90078 or 90079) that includes the B-PER Bacterial Protein Extraction Reagent, DNase I, and Lysozyme.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.