Do I need to do C18 clean-up of my mass spectrometry sample before phosphopeptide enrichment?
Yes. C18 clean-up is necessary to remove small molecules that may interfere with phosphopeptide enrichment. Drying after clean-up is also necessary to concentrate the dilute samples and remove C18 organic eluting solvent so samples can be dissolved in the optimized buffers for phosphopeptide binding. Alternative binding buffers are not recommended as phosphopeptide yield and specificity will be affected. For maximal performance, ensure that the clean, dried peptide samples are completely solubilized in binding buffer with agitation for at least 10 min.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.