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SILAC Protein Quantitation Kit - RPMI 1640 - FAQs

SILAC Protein Quantitation Kit - RPMI 1640 - FAQs

View additional product information for SILAC Protein Quantitation Kit - RPMI 1640 - FAQs (89982)

5 product FAQs found

If I need more of any SILAC Protein Quantitation kit component, can I purchase it separately?

We offer many of the kit components and related alternative components separately. Larger package sizes for SILAC media, dialyzed FBS and heavy and light amino acids are also available.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many samples can be tested with the SILAC Protein Quantitation kit?

Each kit contains 500 mL media, which can isotopically label approximately 1x10E12 cells, depending on cell type.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do I need a NRC license to use the SILAC Protein Quantitation kit?

No, because the heavy isotopes used in the kits are not radioactive.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are the isotopes in the SILAC Protein Quantitation kit radioactive?

No. Deuterated 13C and 15N derivatives of L-arginine, L-lysine and L-leucine are stable heavy isotopes and are therefore not radioactive.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the components of your SILAC Protein Quantitation Kits?

SILAC Protein Quantitation Kits contain all reagents needed for stable isotope labeling with amino acids in cell culture (SILAC) including media, heavy and light amino acids and dialyzed FBS. We offer three classical media kits: Thermo Scientific SILAC Protein Quantitation Kit-RPMI 1640 Medium (Cat. No. 89982), Thermo Scientific SILAC Protein Quantitation Kit-DMEM (Cat. No. 89983) and Thermo Scientific SILAC Protein Quantitation Kit-DMEM F12 (Cat. No. 88439). Each kit includes 2 x 500 mL SILAC media, 1 x 50 mg L-Lysine-2HCl, 1 x 50 mg 13C6 L-Lysine-2HCl, 2 x 50 mg L-Arginine-HCl and dialyzed FBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.