Pierce™ Strong Anion Exchange Spin Column, Mini

Catalog number: 90010

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

Thermo Scientific Pierce Strong Anion Exchange Spin Column, Mini enable rapid protein fractionation and sample preparation based on charge differences.

Features of Strong Anion Exchange Spin Column, Mini:

Fast and simple—membrane-based spin format eliminates column packing
Convenient and expandable—centrifugal format enables convenient processing of multiple samples in parallel
Robust—membrane adsorber spin columns do not crack or run dry
Low bed volume—small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions

Available Formats:
Mini Format: for binding up to 4 mg (500 µL capacity)
Maxi Format: for binding up to 80 mg (20 mL capacity)

Applications:
• Pre-fractionation of proteins in lysate
• Scouting purification conditions for new protein preparation protocols
• Removal of endotoxins from monoclonal antibodies
• Polishing His-tagged proteins after metal chelate chromatography
• Purification and concentration of proteins
• Purification of antibodies from serum, ascites or tissue culture supernatant
• Removal of detergent from protein solutions
• Sample preparation before 1D or 2D PAGE
• Purification of phosphopeptides before MS analysis

Pierce Strong Ion Exchange Spin Columns use membrane-adsorption as a chromatographic method to fractionate proteins based on their charge differences. The matrix has a highly porous structure with pores larger than 3000nm, providing proteins easy access to the membrane's charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.

Ion exchange chromatography separates molecules based on differences in their accessible surface charges. This technique is widely used in the pre-fractionation or purification of a target protein from crude biological samples. Novel membrane-based ion exchange chromatography is attracting attention because of its advantages over resin-based column chromatography. Membrane-based ion exchange chromatography has shorter diffusion times than resin-based chromatography. Interactions between molecules and active sites on the membrane occur in a convective manner through pores, which shortens the diffusion time compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity.

Related Products
Pierce™ Strong Cation Exchange Spin Column, Mini
Pierce™ Strong Cation Exchange Spin Column, Maxi
Pierce™ Strong Anion Exchange Spin Column, Maxi
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Spin Column, Microcentrifuge
Column Type: Ion Exchange, Cellulose Membrane
Product Size: 24 columns
Binding Strength: Strong
Immobilized Phase: Charged Ligand

Contents & storage

Upon receipt store at room temperature.

Documents

Manuals & protocols

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