The GeneChip™ Hybridization, Wash, and Stain Kit is used with the GeneChip Brand Arrays in cartridge format, following the target preparation steps described for 3' eukaryotic, prokaryotic, and exon assays.
For convenience, the Hybridization, Wash, and Stain Kit reagents are formulated into the fewest number of individual components possible, reducing preparation and handling steps. Most of the reagents are ready-to-use solutions.
The kit includes pre-formulated solutions to process 30 arrays on the GeneChip Fluidics Station 400 or 450.
Component Name VolumeBox 1 of 2
Hybridization Module
Pre-Hybridization Module 6 mL
2X Hybridization Mix 4.5 mL
DMSO 0.9 mL
Nuclease-free water 4 mL
Stain Module
- Stain Cocktail 1 18 mL
- Stain Cocktail 2 18 mL
Array Holding Buffer 30 mL
Nuclease-free water 4 mL
Box 2 of 2
Wash Buffer A* 3 bottles, 800 mL/bottle
Wash Buffer B* 1 bottle, 600 mL/bottle
*Wash Buffer A and Wash Buffer B are also available for purchase individually.
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