GeneChip™ Wash Buffer A - FAQs

GeneChip Wash Buffer A can be kept at room temperature (overnight) the day before the experiment. If you see crystals, we recommend equilibrating the buffer up to 4 hrs (if no external heating is used) until it reaches room temperature. You can also vortex it with a stir bar at room temperature.

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The DNA Labeling Reagent has about 12 months of dating from the date of shipment.

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The GeneChip HT hybridization, Wash Stain Kit (Cat. No. 901219) is part of an older kit that is discontinued and no longer available.
The kit consisted of:
- HT Pre-Hyb mix, HT 1.2X Hyb mix, HT Stain Cocktail 1& 3, HT Stain Cocktail 2, HT Wash Buffers A, HT Wash Buffer B, and HT Array Holding Buffer.

The GeneChip HWS kit (Cat. No. 900720) is a current product that can be ordered.
This kit consists of:
- Pre-Hyb module, 2X Hyb mix, DMSO, Nuclease Free Water, Stain Module (Stain Cocktail 1 and Stain Cocktail 2) Wash Buffers, Wash Buffer B, and Array Holding Buffer.

The HT kit was formulated for high throughput usage. Therefore, the formulations differ. The Wash buffers A and B are similar, but they have slight variations in terms of additives.
The Hyb mixes have different concentrations. The stain cocktails have different stain mixtures (Stain 1 & Stain 3 vs Stain 1).
Therefore, we do not recommend swapping any of these reagents.
Only the Array Holding Buffer is the same and could be swapped.
The exact components and concentrations of the reagents are proprietary information and thus cannot be disclosed.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.