901942 - FAQs

To install library files for the Gene 2.0 ST Array for Command Console, simply run the executable library file installer.

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Installing the library files for the Gene 1.0 ST Array for AGCC requires the use of the Affymetrix Library File Importer which converts GCOS library files into AGCC format, then imports the files to the Command Console library file folder. The Library File Importer can convert files from the local GCOS installation, from a GCOS server on a remote Windows Server, or from a GCOS library file package on a CD or disk drive.

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The Gene 2.0 ST Array is a 100 format array.

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The GeneChip Gene 1.0 ST Array is an 81/4 format. Please note that 81/4 format is to be treated equivalent to 100 format arrays.

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FS450_00002 should be used when processing Gene 2.0 ST Array. Up-to-date fluidics scripts can be obtained from the website.

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FS450_00007 should be used when processing Gene 1.0 ST Array. Up-to-date fluidics scripts can be obtained from the website.

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GeneChip Operating Software (GCOS) or GeneChip Command Console Software (AGCC) can be used to process Gene 1.0 ST Arrays through fluidics and scanning. Gene 2.0 ST Arrays utilize an updated installer package that is not compatible with GCOS. Gene 2.0 ST Arrays require AGCC for fluidics and scanning. Affymetrix Expression Console Software is required to perform QC analysis of q4Gene 1.0 ST and Gene 2.0 ST Array data. Expression Console Software can be downloaded free from the website.

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Using the Ambion WT Expression kit, a minimum of 50 ng of starting total RNA is required. No ribosomal RNA reduction of the starting material is required.

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The WT Plus kit or the WT Pico kit is recommended for preparing samples for use with the Gene 1.0 ST Array and the Gene 2.0 ST Array.

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