GeneChip™ Mouse Gene 1.0 ST Assay, 10 samples - FAQs

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3 product FAQs found

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.

Is the protocol same for Exon 1.0 St and Gene 1.0 St arrays?

The protocols for the Gene 1.0 ST and Exon 1.0 ST arrays are the same until the fragmentation and labeling steps.
Depending on the input amount, please refer to the GeneChip WT Pico Reagent Kit or the GeneChip WT PLUS Reagent Kit for the protocol:
- WT Pico: 50 - 500ng of total RNA
- WT PLUS: ≥ 100pg of total RNA (approx. 10 cells)
From the hybridization step onwards, the differences in protocol are due to the format of the arrays. The Exon array is a 49 format array, whereas the Gene 1.0 ST array is a 169 format array.
For the fluidics step the following scripts should be used depending upon which array you are using:
- Gene 1.0 St array: FS450_0001
- Exon 1.0 St array: FS450_0007

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.