Comprehensive design We understand that rats are an important model organism commonly used for studying human disease. In order to keep your experiments relevant to the understanding of the transcriptome, we have designed an array based on the most recent genome content for generating genome-wide expression profiles.
The GeneChip™ Rat Gene 1.0 ST Arrays and GeneChip WT PLUS Reagent Kit offers a unique feature not available in other microarrays. Unlike traditional array designs that rely on a probe(s) designed to the first exon of the gene's 3' end, the hundreds of thousands of probes in this array are designed to every exon of every transcript represented on the array.
The high transcript coverage (median of 22 probes per gene) yields accurate detection for genome-wide transcript expression changes. These arrays provide higher resolution and accuracy than many of the classical 3'-biased microarray solutions available. The whole-transcript analysis approach enables researchers to detect multiple transcript isoforms from a given gene, including those that could be missed using a 3'-biased expression design, such as splice variants, non-polyadenylated transcripts, transcripts with alternative polyadenylation sites, and truncated transcripts.
Key benefits of Rat Gene 1.0 ST Arrays • Whole-transcript analysis enables the capture of transcript isoforms you may miss with 3'-biased expression designs • Comprehensive transcriptome coverage provides the best opportunity to discover interesting biology: - >27,000 protein coding transcripts - >24,000 Entrez genes • Measure alternative splicing events/transcript variants with probes designed to maximize coverage of exons • Reproducible: Signal correlation coefficient ≥ 0.99
Content profile of Rat Gene 1.0 ST Arrays Since the design of the GeneChip Rat Gene 1.0 ST Array, there has been a substantial increase in the structural and functional understanding of the rat genome. In order to provide the research community with a tool that can measure the differential expression of this exciting class of RNA transcripts, we designed the GeneChip Rat Gene 2.0 ST Array (sold separately).
GeneChip WT PLUS Reagent Kit The WT PLUS Reagent Kit uses a reverse transcription priming method that primes the entire length of each RNA transcript, including both poly-A and non-poly-A mRNA to provide complete and unbiased coverage of the transcriptome. The kit efficiently generates amplified and biotinylated sense-stranded DNA targets, avoiding loss of specificity due to antisense strand interference.
The WT PLUS Reagent Kit includes the amplification module coupled with GeneChip WT Terminal Labeling and Controls Kit for preparing hybridization-ready targets from 50 ng to 500 ng of total RNA.
Features and benefits: • A complete target prep kit with proven sensitivity and specificity resulting from an optimized design for WT arrays • A low sample input requirement of 50 ng. The kit works with a variety of sample types, including fresh-frozen tissues, cell lines, and whole blood • StreamLined workflow with no ribosomal RNA depletion requirement or a globin reduction step for blood samples • Opportunities to bundle WT arrays with WT reagents, offering exceptional cost savings • Convenient single source for ordering and support
For Research Use Only. Not for use in diagnostic procedures.