I used the BrdU Staining Kit and got very high background staining? What could have caused this?
Here are possible causes for high background staining:
1. Tissue may require a longer blocking step.
2. Inadequate rinsing of slides.
3. Deparaffinization was not complete.
4. Over-development of substrate may have occurred.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
1. Tissue may require a longer blocking step.
2. Inadequate rinsing of slides.
3. Deparaffinization was not complete.
4. Over-development of substrate may have occurred.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.