MM BAC CLONE - FAQs

Which Mouse BAC Libraries does Thermo Fisher Scientific offer, and how can you find specific gene within a BAC clone?

For Mouse BACs, we offer a CITB Mouse library and 2 BAC libraries from Roswell Park. Below are the details:

The CITB (California Institute of Technology BAC) Mouse BAC was constructed from the ES cell line/ CJ7/129Sv. The average insert size is 130 Kb. The clones for the Mouse BAC library were constructed with the pBeloBAC11 vector. This library consists of 612 384-well plates.

RPCI-22 Female Mouse BAC Library: The RPCI-22 (Roswell Park Cancer Institute) BAC (Bacterial Artificial Chromosome) Library is derived from female 129S6/SvEvTac (Taconic) mouse spleen genomic DNA. Average insert size is 155 Kb, with a maximum of 300 Kb.

RPCI-23 Female Mouse BAC Library: The RPCI-23 (Roswell Park Cancer Institute) BAC (Bacterial Artificial Chromosome) Library was constructed from pooled tissues derived from three female C57BL/6J mice. Average insert size is 197kb.

For help identifying RPCI -23 clones you can search the Mouse Screening Project for Mouse Marker/Clone Search at the following website: http://genomics.roswellpark.org/mouse/overview.html. This site can be used to retrieve clone or marker information from the Mouse Screening & Fingerprinting Project database. The search system allows the user to enter a particular clone or marker name. For locating a specific BAC clone you can also search literature, NCBI, the above websites and the Ensembl Genome Browser for more information.   

How do I find/order an individual BAC/PAC clone?

There are several internet sites that may be used to locate BAC/PAC clones. One of them is listed below:

- Go here.
- Select the species of interest from the “Select organism” box.
- Type the feature, location or gene name in the “Search in genome” box on the right.
- As an example, choose “Search in genome”, enter “ITGA5” as the “Feature name” and click the search icon.
- Click on “Tracks shown”.
- Find the Clone library of interest, click on “Genomic Clones” and then select the box for the library of interest: for example: RP23 (RPCI-23 Female (C57BL/6J) Mouse BAC Library) and click “Configure”.
- In the refreshed window, you will see a list of BAC/PAC clones containing the ITGA5 gene (for example: RP23-427P19 where the Clone ID is 427P19).
- Go to CloneRanger, select the “BAC Mouse RP23” Collection, type in the Clone ID (in this case, 427P19), and click “Submit” to see if we carry the BAC clone. If the order is being placed by phone, provide the Cat No. (RPCI23.C) and the Clone address (in this case, RP23-427P19).