CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit

Catalog number: A1024101

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture

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Description

Get the most from your fed-batch cultures
The CHO CD EfficientFeed™ Kit is a system designed to help you more quickly and efficiently identify a feed supplement formulation that will improve fed-batch productivity 2- to 5-fold versus batch conditions, as well as identify a feed supplement formulation that will help develop bioreactor cell seed stocks more quickly.

The liquid format is designed for small scale use and experimentation. EfficientFeed™ A & B are also available in AGT™ format (CHO CD EfficientFeed™ A AGT™ and CHO CD EfficientFeed™ B AGT™ Nutrient Supplements) for large scale manufacturing and ease of use.
• Demonstrated 2- to 5-fold increases in productivity
• Identification of feed for growing cells faster for bioreactor seeding
• Chemically defined - no growth factors, lysates, or less consistent hydrolysates needed
• Liquid supplements made from AGT™ powders for simplified transition to larger scales

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Two benefits from one Kit
The CHO CD EfficientFeed™ Kit contains two feed supplements, A and B, based on successful chemically defined media formulations that you may already be using or considering for your batch culture medium (CD OptiCHO™ Medium or CD CHO Medium). Built using chemically defined components in higher concentrations, and based on typical CHO culture needs, these feeds have demonstrated the capacity to increase the production of proteins and antibodies by as much as 5-fold versus batch culture conditions. Gain increased productivity from one formulation and higher cell growth kinetics from the other, or a combination of the two. Higher cell growth kinetics can provide quicker development of cells for seeding larger-scale bioreactors. Being able to load the bioreactor more quickly may allow you to increase the number of bioreactor runs per year. The kit provides a complete decision workflow, and set of proposed protocols to help you choose the most efficient path towards feed identification.

Both CHO CD EfficientFeed™ A and B are chemically defined, membrane-filtered liquids reconstituted from AGT™ powders plus supplements. They contain a carbon source, concentrated amino acids, vitamins, and trace elements. They are free of lipids, vegetable hydrolysates, and growth factors. Both supplements can boost cell growth and productivity, either alone or in combination with the other.

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figure 1a & 1b). Furthermore, they outperform competitive alternatives, including less consistent hydrolysates (Figure 2, 3a & 3b).

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocessing settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ A AGT™ Kit that is a two part kit that is primarily AGT dry format materialis offered in 10 L and 100 L packaging that allows scale-up from the 1 L liquid supplement as a seamless process, as well as allow for cost savings when purchasing production level volumes. CHO CD EfficientFeed™ B AGT™ nutrient supplement is in a single part AGT dry format that is seamless for scale-up in 10 L and 100 L sizes as well.

Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ A & B supplements (Figures 4-5). Both forms have performed similary in our testing, and the AGT™ version has been used successfully in many processes by multiple companies.

AGT™ Format Ease of Use
CHO CD EfficientFeed™ A AGT™ Kit Nutrient Supplement delivers dry format scale-up convenience as well as simple reconstitution with approximately 1% concentrated solution add back. CHO CD EfficientFeed™ B AGT™ Nutrient Supplement delivers dry format scale-up convenience as well as simple reconstitution in a single part AGT dry format. For more details, consult the supporting documents provided on those product pages.

For research or further manufacturing use. Not for use in diagnostic procedures.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Format: Bottle(s)
Comments: Chemically Defined Nutrient Feeding Supplement
Cell Line: CHO
Glutamine: No Glutamine
Sterility: Sterile
Serum Level: Serum-Free
Culture Type: Suspension Cell Culture
Product Size: 1 kit
Classification: Animal Origin-Free, Serum-Free, Protein-Free, Chemically Defined
Endotoxin Level: Low
Other Additives: No Insulin
Tests Performed: pH
Volume (Metric): 2 L
Included Antibiotics: No antibiotics
Phenol Red Indicator: No Phenol Red
Purity or Quality Grade: Cell Culture Grade, Research Grade, Qualified for Further Manufacturing
Sodium Pyruvate Additive: Sodium Pyruvate
Sodium Bicarbonate Buffer: Sodium Bicarbonate

Contents & storage

One liter each of CHO CD EfficientFeed™ A and CHO CD EfficientFeed™ B, and a manual.
Store CHO CD EfficientFeed™ A and CHO CD EfficientFeed™ B at +2–8°C in the dark.

Documents

Manuals & protocols

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