BrightComp eBeads™ Compensation Bead Kits, 25 tests

BrightComp eBeads™ Compensation Bead Kits, 25 tests - FAQs

View additional product information for BrightComp eBeads™ Compensation Bead Kits - FAQs (A54743, A54741, A54742, A10514, A54740)

3 product FAQs found

Can I use the GFP BrightComp eBeads to compensate for FITC or Alexa Fluor 488?

No. You must always compensate with the same fluorophore present on the samples. The GFP BrightComp eBeads Compensation Bead Kit spectrally emulates GFP and must only be used on GFP-expressing samples.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I used the GFP BrightComp eBeads Compensation Bead Kit. My peaks are broader than what I see in the manual (Figure 1). What is causing this?

This may be caused by aggregation of the beads. Mix thoroughly before dispensing a drop from the stock vial and mix again after adding buffer. Aggregation may be also be caused by microbial contamination or if the beads were frozen. In these cases, they can no longer be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What material are the beads in the GFP BrightComp eBeads Compensation Bead Kit composed of?

These are polystyrene beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.