Informational:Inquire about OEM or Commercial Supply version of this product here.
Informational:This product has been superseded by the RiboMinus™ Eukaryote System v2 and Low Input RiboMinus™ Eukaryote Kit v2 and will be discontinued in the near future.
Invitrogen™

RiboMinus™ Eukaryote Kit for RNA-Seq

Catalog number: A1083708
Invitrogen™

RiboMinus™ Eukaryote Kit for RNA-Seq

Catalog number: A1083708
Catalog Number
A1083708
also known as A10837-08
Unit Size
8 Preps (10 µg⁄prep)
Price (USD)
Price: 890.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1083708
also known as A10837-08
8 Preps (10 µg⁄prep)
Price: 890.00
Your price:
-
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The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:

• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Magnetic Bead
Sample Type
Total RNA
Final Product Type
Transcriptome RNA
High-throughput Compatibility
High-throughput Compatible
For Use With (Application)
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
No. of Reactions
8 Preps
Product Line
RiboMinus™
Quantity
1 Each
Sequencing Type
Transcriptome Sequencing
Shipping Condition
Wet Ice
Starting Material Amount
2-10 μg Total RNA
Target
Transcriptome RNA
System Type
RiboMinus
Yield
1 μg

Contents & Storage

Each kit contains:
• 6 mL RiboMinus™ Magnetic Beads
• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)
• 10 mL Hybridization Buffer
• 12 mL DEPC-treated (RNase-free) water

Store at 4°C.

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