Jump-In™ Fast Gateway® Core Kit

Catalog number: A10894

Invitrogen™  Related applications: Protein Expression

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Description

The Jump-In™ Fast Gateway® Core Kit is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway® Core Kit contains the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
Please note: the pJTI™ Fast DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway® Pro Kits.
This kit is also a component of the Jump-In™ Fast Gateway® System.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: None (Promoterless)
Key Function: Clone your own Promoter, Stable Cell Line Development, Targeted Integration
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Hygromycin
Vector Type: Jump-In Vectors, pJTI
Expression: Mammalian
High Throughput Compatibility: High Throughput-Compatible
Product Size: 1 kit
Selection Marker (Eukaryotic): HygroR

Contents & storage

The Jump-In™ Fast Gateway® Core Kit is composed of 2 vectors:
pJTI™ Fast DEST Vector (150 ng⁄µl; total 40 µl),
pJTI™ PhiC31 Int Vector (0.5 µg⁄µl; total 20 µl).

Store vectors at -20°C.

Documents

Manuals & protocols