pEBNA DEST Vector Kit

Catalog number: A10898

Invitrogen™  Related applications: Protein Expression

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The promoter-less pEBNA DEST expression vector is an episomal vector that permits extra-chromosomal (episomal) replication in mammalian cells. Episomal replication is facilitated by an EBV origin of replication (EBV-oriP) and a nuclear antigen, EBNA-1. Episomal replication enables transient, high level expression of a gene of interest.

Please note: the pEBNA DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway® Pro kits.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: None (Promoterless)
Key Function: Episomal Expression, Clone your own Promoter, Stable Cell Line Development
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Hygromycin
Vector Type: pDEST
Cleavage: No Cleavage Site
DNA Yield: High copy number
PCR Enzyme: Taq Polymerase, Proof-Reading Polymerase
Plasmid Type: High Copy Plasmid
Product Size: 6 µg
Directionality: Yes
Gateway Enzyme: LR Clonase® II, LR Clonase® II Plus
Volume (Metric): 40 µl
Recognition Site: TK polyA reverse priming site
Cloning Efficiency: 95 %
Replication Origin: oriP, pUC Origin
Selection Marker Promoter: PGK Promoter
Selection Marker (Eukaryotic): HygroR
Constitutive or Inducible System: Constitutive
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)

Contents & storage

pEBNA DEST (150 ng⁄µl; total: 40 µl)

Store pEBNA DEST at -20°C.


Manuals & protocols