Gibco™

CHO DG44 Cells (cGMP banked) and Media Kit

Das CHO DG44 Cells (cGMP Banked) and Media-Kit ist für das Wachstum von Dihydrofolatreduktase-defizienten (DHFR-) Chinese Hamster Ovary (CHO)-Zellen undWeitere Informationen

Katalognummer	Menge
A1100001	1 Kit

Katalognummer A1100001

Preis (EUR)

11.500,00

Menge:

1 Kit

Preis (EUR)

11.500,00

Das CHO DG44 Cells (cGMP Banked) and Media-Kit ist für das Wachstum von Dihydrofolatreduktase-defizienten (DHFR-) Chinese Hamster Ovary (CHO)-Zellen und die Expression rekombinanter Proteine in Suspensionskulturen optimiert.Lieferumfang des Kits:

Gibco™ CD DG44 Medium—chemisch definiertes Medium ohne Proteine, das speziell für optimales Wachstum und optimale Expression von DG44-Zellen in Suspensionskulturen ntwickelt wurde.

eCHO DG44-Zellen (cGMP Banked)—CHO DG44-Mutterzellen wurden auf Einhaltung der cGMP-Qualitätsstandards hergestellt, gebankt und getestet.Sie sind auf das CD DG44-Medium vorangepasst und für überragendes Zellwachstum und Transfektionseffizienz selektiert.

Darüber hinaus enthält das CHO DG44 Cells (cGMP Banked) and Media-Kit L-Glutamin und Pluronic™ F-68 für optimales Wachstum und die Proteinexpression von DG44-Zellen in einer chemisch definierten Umgebung ohne Proteine.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

KlassifikationOhne Stoffe tierischen Ursprungs, Chemisch definiert, Protein-frei, Serum-frei

MedienempfehlungCD DG44 Medium

Anzahl Zellen1 x 10⁷

ProduktlinieGibco

Menge1 Kit

ZelllinieCHO DG44

Culture TypeSuspensions-Zellkultur

SpeziesHamster

Unit SizeEach

Inhalt und Lagerung

CHO DG44 Cells (cGMP banked) and Media Kit (SKU A1100001) contains:

• CD DG44 Medium 1000 mL- 12610-010, store in the dark at 2-8°C
• CHO DG44 Cells (cGMP banked) 1 vial (> 1x10^7 cells) - A1097101 (see limited label license), store in liquid nitrogen
• PLURONIC F-68, 10% (100X) 100 mL - 24040-032, store at room temperature
• L-glutamine-200mM (100X) 100 mL - 25030-081 or 25030-024 (CE marked version for Europe only), store at -20°C

Häufig gestellte Fragen (FAQ)

Has the DG44 cell line been used for the large scale production of any FDA-approved biotherapeutic proteins?

70% of all therapeutics produced in mammalian cells are expressed in CHO cells. CHO cells are preferred because of human-like glycosylation and no propagation of human-like viruses. Most therapeutic IgGs are produced using the CHO platform and a number of FDA-approved biotherapeutics have been produced in DG44 cells.

Why are the defrosted CHO DG44 Cells (cGMP banked) transferred directly into pre-warmed media instead of being centrifuged for recovery when using the CHO DG44 Cells (cGMP banked) and Media Kit (Cat. No. A1100001)?

The defrosted contents of the vial of Gibco CHO DG44 Cells are transferred directly to 29 mL of pre-warmed media and then incubated because the cells are extremely fragile upon recovery from cryopreservation. Centrifuging the cells at this stage could cause significant damage, reducing their viability and recovery efficiency. To help ensure optimal recovery and viability, the cells are directly transferred to pre-warmed media and incubated under optimal conditions. This approach is also why we do not mention centrifuge resuspension in the subculture section. We have found that the shear forces during centrifugation are more harmful to the cells than retaining a small amount of old media or DMSO. The volume of media in the flask has been found to sufficiently dilute the DMSO such that it does not impact recovery or cell growth rather than using a centrifuge.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I learn more about commercial licensing of Freedom kits, ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

To inquire about a Commercial Production or Service License, please email: outlicensing@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the term of the license agreement for ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

Licensing rights are granted in perpetuity for the Commercial Production License. The term of the Commercial Service License is negotiated between the parties.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is there a pull-through royalty on final products or an annual maintenance fee for using ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)??

Only a one-time license fee is required for a Commercial Production License, and it covers use during the clinical trial process and commercialization of an unlimited number of molecules.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all CHO DG44 Cells (cGMP banked) and Media Kit FAQs