CHO DG44 Cells (cGMP banked) and Media Kit

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Description

The CHO DG44 Cells (cGMP banked) and Media Kit is optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. The kit provides:

GIBCO® CD DG44 Medium—a chemically defined, protein-free medium specifically designed to enable optimal growth and expression of DG44 cells in suspension culture

CHO DG44 cells (cGMP banked) —Parental CHO DG44 cells have been produced, banked and tested to meet cGMP quality standards. They are pre-adapted to CD DG44 Medium and selected for superior cell growth and transfection efficiencies.

In addition, the CHO DG44 Cells (cGMP banked) and Media Kit includes L-glutamine and Pluronic® F-68 for optimal growth and protein expression of DG44 cells in a protein-free, chemically defined environment.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Hamster
Cell Line: CHO DG44
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Culture Type: Suspension Cell Culture
Endotoxin Level: Low
Glutamine: No Glutamine
Included Antibiotics: No antibiotics
Media Adaptation: CD DG44 Medium
Media Recommendation: CD DG44 Medium
Number of Cells: 1 x 107
Parental Cells: DG44
Phenol Red Indicator: No Phenol Red
Product Size: 1 kit
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
Stably Integrated Gene or Element: dhfr-
Supplements: Hypoxanthine, Thymidine

Contents & storage

CHO DG44 Cells (cGMP banked) and Media Kit (SKU A1100001) contains:

• CD DG44 Medium 1000 mL- 12610-010, store in the dark at 2-8°C
• CHO DG44 Cells (cGMP banked) 1 vial (> 1x10^7 cells) - A1097101 (see limited label license), store in liquid nitrogen
• PLURONIC F-68, 10% (100X) 100 mL - 24040-032, store at room temperature
• L-glutamine-200mM (100X) 100 mL - 25030-081 or 25030-024 (CE marked version for Europe only), store at -20°C

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