Get out of the cold & warm up to new Gibco BenchStable Media! Optimized for storage at room temperature »

Blasticidin S HCl (10 mg/mL)

Catalog number:  A1113902

 Related applications:

Insect Cell Culture

| Mammalian Cell Culture

| Transfection

| Transformation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome. Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 2 to 20 µg/ml depending on the cell line. This product is provided as a solution at 10 mg/ml in 20 mM HEPES (pH 7.2-7.5). Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications.

In addition, Blasticidin S is used as a bacterial selective antibiotic in the concentration range of 50–100 µg/ml. Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD (2) or bsr.These deaminases convert Blasticidin S to a nontoxic derivative. For selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S. Life Technologies also offers a variety of selection antibiotics.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing
Gibco® Blasticidin S HCl is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.


Validated Application: Bacterial Selection, Eukaryotic Selection⁄Stable Cell Line Generation
Agent: Blasticidin
Form: Liquid
Reagent Type: Antibiotic (Selective)
Concentration: 10 mg⁄ml
Formula Weight: 458.9 g⁄mole
Product Line: Gibco®
Product Size: 20 mL
Quantity: 1 bottle
Sterility: Sterile
Shipping Condition: Dry Ice

Contents & storage

Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Frozen
Shelf life: 9 months from date of manufacture


Manuals & protocols