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Gibco™

Blasticidin S HCl (10 mg/mL)

Catalog number:  A1113902

Related applications:

Insect Cell Culture

| Mammalian Cell Culture

| Transfection

| Transformation

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Description

Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome. Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 2 to 20 µg/ml depending on the cell line. This product is provided as a solution at 10 mg/ml in 20 mM HEPES (pH 7.2-7.5). Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications.

In addition, Blasticidin S is used as a bacterial selective antibiotic in the concentration range of 50–100 µg/ml. Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD (2) or bsr.These deaminases convert Blasticidin S to a nontoxic derivative. For selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S. Life Technologies also offers a variety of selection antibiotics.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing
Gibco® Blasticidin S HCl is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Agent: Blasticidin
Concentration: 10 mg⁄ml
Form: Liquid
Formula Weight: 458.9 g⁄mole
Product Line: Gibco®
Product Size: 20 mL
Quantity: 1 bottle
Reagent Type: Antibiotic (Selective)
Shipping Condition: Dry Ice
Sterility: Sterile
Validated Application: Bacterial Selection,Eukaryotic Selection⁄Stable Cell Line Generation

Contents & storage

Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Frozen
Shelf life: 9 months from date of manufacture