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Gibco™

Blasticidin S HCl (10 mg/mL)

Catalog number: A1113902
Gibco™

Blasticidin S HCl (10 mg/mL)

Catalog number: A1113902
Catalog Number
A1113902
also known as A11139-02
Unit Size
20 mL
Price (USD)
794.00
Your Price:
Availability
***
Quantity
Catalog Number
A1113903
also known as A11139-03
Unit Size
10 x 1 mL
Price (USD)
397.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1113902
also known as A11139-02
20 mL
794.00
Your Price:
***
A1113903
also known as A11139-03
10 x 1 mL
397.00
Your Price:
***
Product Overview
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Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase. For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.

Applications
View detailed protocols on blasticidin selection in mammalian cells, E. coli, and yeast.

Specifications

Concentration
10 mg⁄ml, 10 mg⁄mL, 10 mg⁄ml
Product Type
Selective Antibiotic
Sterility
Sterile-filtered
Agent
Blasticidin
Formula Weight
458.9 g⁄mole
Product Line
Gibco™
Form
Liquid
Shelf Life
9 Months
Reagent Type
Blasticidin S HCL
Cell Type
Eukaryotic Cell, Prokaryotic Cell
Culture Type
Mammalian, Insect

Contents & Storage

Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture

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