Invitrogen™

ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System

Catalog number: A11141
Related applications: Protein Expression
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Invitrogen™

ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System

Catalog number: A11141
Related applications: Protein Expression
Technical SupportCustomer Service
Catalog Number
A11141
Unit Size
20 reactions
Price (USD)
4,530.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1114120 reactions
4,530.00
Your Price:
***
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The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features
• WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoter

Kit Includes
• ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat # A11144)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
• Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat # 12538120)
• One Shot™ Stbl3™ Chemically Competent E. coli (Cat # C737303)
• Geneticin™ (Cat # 10131035)
• pENTR™ Gus positive control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance Bacterial
Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method
Gateway™
Constitutive or Inducible System
Inducible
Delivery Type
Lentiviral
For Use With (Application)
Protein Expression
For Use With (Equipment)
Gateway™, HiPerform™, T-REx™, ViraPower™
Gateway Enzyme
LR Clonase™ II Plus, LR Clonase™ II
Inducing Agent
Doxycycline, Tetracycline
Key Functions
Viral Expression
No. of Reactions
20
PCR Enzyme
Proof-Reading Polymerase
Product Line
Gateway™, HiPerform™, T-REx™, ViraPower™
Product Type
Mammalian Expression system
Protein Tag
V5 Epitope Tag
Quantity
20 Reactions
Replication Origin
pUC Origin
Selection Agent (Eukaryotic)
Blasticidin
Selection Marker Eukaryotic
BsdR
Selection Marker Promoter
PGK Promoter
Special Elements
WPRE, cPPT
Cell Type
293-FT
Format
Kit
Promoter
CMV⁄TO
Vector
pLenti

Contents & Storage

Contents

The ViraPower™ HiPerform™ T-Rex™ Gateway™ Expression System includes the following components:
• ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat # A11144) which includes:
  • 6 ug of pLenti6.3⁄TO⁄V5-DEST vector (40 µL of vector at 150 ng⁄µL in TE Buffer, pH 8.0)
  • 20 ug of pLenti3.3⁄TR vector (40 µL of vector at 0.5 µg⁄µL in TE Buffer, pH 8.0)
  • 10 ug of pLenti6.3⁄TO⁄V5-GW⁄lacZ plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)
  • 1 ml of Tetracycline (10 mg⁄mL in water)

    • • ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000) which includes:
  • 195 ug of ViraPower™ Lentiviral Packaging Mix (Contains 195 uL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1 ug⁄uL in TE Buffer, pH 8.0)
  • 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)
  • 50 mg of Blasticidin powder (Cat # R21001)

    • • 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106 frozen cells in 1 mL of Freezing Medium.
    • Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat # 12538120): 40 uL of Gateway™ LR Clonase™ II Plus Enzyme Mix and 40 uL of Proteinase K Solution at a concentration of 2 ug⁄mL.
    • One Shot™ Stbl3™ Chemically Competent E. coli (Cat # C737303): 21 X 50 uL of Stbl3™ Cells, 6 mL of S.O.C. Medium and 50 uL of pUC19 control DNA (10 pg⁄uL).
    • Geneticin™ (Cat # 10131035): 20 mL of Geneticin™ solution (50 mg⁄mL).
    • pENTR™ Gus positive control plasmid

    Storage

    • Vectors: -20°C
    • ViraPower™ Packaging Mix: -20°C
    • LR Clonase™ II Plus Enzyme Mix: -20°C for storage up to 6 months, -80°C for long term storage
    • Tetracycline: -20°C (protected from light)
    • Blasticidin: -20°C
    • Geneticin: 4°C or -20°C
    • Lipofectamine™ 2000: 4°C (do not freeze)
    • One Shot™ Stbl3™ Chemically Competent E.coli: -80°C
    • 293 FT Cells: Liquid Nitrogen
    • pENTR™ Gus positive Control: -20°C

    Figures