Oil Red O - FAQs

How do I prepare a stock solution and working solution of Oil Red O for staining?

We recommend the following instructions to prepare a stock or working solution of Oil Red O:

Oil Red O Stock Solution
1. Dissolve 300 mg of Oil Red O in 100 mL of 100% isopropanol.
2. Mix well and leave undisturbed for 20 minutes to make the Oil Red O Stock Solution.
The Oil Red O stock solution is stable for 1 year.

Oil Red O Working Solution
1. Add 3 parts of Oil Red O Stock Solution (30 mL) to 2 parts of water (20 mL).
2. Mix well and leave undisturbed for 10 minutes.
3. Filter through Whatman No. 1 filter paper.
The Oil Red O working solution is stable for 2 hours and must be prepared 15 minutes before use.